Our biochemists and cell biologists use their expertise to develop and run new high throughput biological assays used to identify food components that interact with inflammatory pathways.
The cell based models used in our systems include two NOD variants, a TNF-alpha promoter variant and IL-10 variants, all of which are genetic variants found in our IBD populations as well as in other inflammatory diseases. We also have a JAK2 based enzyme assay, with Time Resolved Fluorescence and Fluorescence Resonance Energy Transfer (TR-FRET) read out to minimize interference from other food components.
Ideally we are looking for compounds that ‘cure’ a genetic defect, but we also identify general anti-inflammatory compounds. All the bioassay results are stored on a searchable database, so that we can rapidly examine the specifity of activity of hundreds of food extracts and their bioactive components.